Fast immobilization of human carbonic anhydrase II on Ni-Based metal-organic framework nanorods with high catalytic performance
Jiao, M., He, J., Sun, S., Vriesekoop, F., Yuan, Q., Liu, Y. and Liang, H. (2020) Fast immobilization of human carbonic anhydrase II on Ni-Based metal-organic framework nanorods with high catalytic performance. Catalysts, 10 (4).
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Abstract
Carbonic anhydrase (CA) has received considerable attention for its ability to capture carbon dioxide efficiently. This study reports a simple strategy for immobilizing recombinant carbonic anhydrase II from human (hCA II) on Ni-based MOFs (Ni-BTC) nanorods, which was readily achieved in a one-pot immobilization of His-tagged hCA II (His-hCA II). Consequently, His-hCA II from cell lysate could obtain an activity recovery of 99% under optimal conditions. After storing for 10 days, the immobilized His-hCA II maintained 40% activity while the free enzyme lost 91% activity. Furthermore, during the hydrolysis of p-nitrophenyl acetic acid, immobilized His-hCA II exhibited excellent reusability and still retained more than 65% of the original activity after eight cycles. In addition, we also found that Ni-BTC had no fixation effect on proteins without histidine-tag. These results show that the Ni-BTC MOFs have a great potential with high efficiency for and specific binding of immobilized enzymes. V
| Item Type: | Article |
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| Keywords: | one-pot hydrothermal, immobilizing recombinant, His-hCA II, Ni-BTC nanorods |
| Divisions: | Food, Land and Agribusiness Management |
| Depositing User: | Ms Kath Osborn |
| Date Deposited: | 15 May 2020 15:45 |
| Last Modified: | 22 Jul 2021 11:30 |
| URI: | https://hau.repository.guildhe.ac.uk/id/eprint/17544 |
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