Effects of exogenous abscisic acid and gibberellic acid on pre-maturity α-amylase formation in wheat grains

Kondhare, K.R., Kettlewell, P.S., Farrell, A.D., Hedden, P. and Monaghan, J.M. (2012) Effects of exogenous abscisic acid and gibberellic acid on pre-maturity α-amylase formation in wheat grains. Euphytica, 188 (1). pp. 51-60.

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Abstract

Effects of in situ and in vitro applied abscisic acid (ABA) and gibberellic acid (GA3) on pre-maturity α-amylase (PMA) were investigated in glasshouse experiments under cool-temperature shock-induced and non-induced conditions using UK winter wheat varieties, Spark (low PMA susceptible) and Rialto (high PMA susceptible). In the in situ study, hormone solutions (ABA [100 μM], GA3 [50 μM] and ABA + GA3 [100 + 50 μM]) were applied during mid-grain development to intact, developing grains of induced and non-induced plants. Alpha-amylase activity was measured in embryoless half-grains at maturity by a modified Phadebas assay. In the in situ study under non-induced conditions, applied ABA had no significant effect on α-amylase in either variety whereas applied GA3 significantly increased α-amylase in Rialto, but not in Spark. In the in situ study under induced conditions, applied ABA and GA3 produced no significant effect on α-amylase in Spark; however in Rialto, applied ABA produced a small but significant decrease in α-amylase whereas GA3 significantly increased α-amylase under induced conditions. In the in vitro study, spikes were harvested from induced/non-induced plants at 44/40 days after anthesis. Embryoless half-grains were incubated in hormone solutions at 25 °C and α-amylase activity was measured. Results similar to the in situ study were observed in the in vitro study for both varieties and conditions, except GA3 treatment which significantly increased α-amylase under non-induced conditions in Spark. From these exogenous hormone studies, it appears that GA-response is a major factor during PMA induction by a cool-temperature shock whereas ABA-response seems to be of less importance.

Item Type: Article
Divisions: Crop and Environment Sciences
Depositing User: Mr Darren Roberts
Date Deposited: 08 Nov 2018 15:12
Last Modified: 29 Nov 2018 12:12
URI: http://hau.repository.guildhe.ac.uk/id/eprint/16980

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