Effects of varying doses of Β-nerve growth factor on the timing of ovulation, plasma progesterone concentration and corpus luteum size in female alpacas

Stuart, C.C., Vaughan, J.L., Kershaw, C.M., Wilkinson, J., Bathgate, R. and De Graaf, S.P. (2014) Effects of varying doses of Β-nerve growth factor on the timing of ovulation, plasma progesterone concentration and corpus luteum size in female alpacas. Reproduction, Fertility and Development, 27 (8). pp. 1181-1186.

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Abstract

Ovulation in camelids is induced by the seminal plasma protein ovulation-inducing factor (OIF), recently identified as β-nerve growth factor (β-NGF). The present study measured the total protein concentration in alpaca seminal plasma using a bicinchoninic acid (BCA) protein quantification assay and found it to be 22.2 ± 2.0 mg mL–1. To measure the effects of varying doses of β-NGF on the incidence and timing of ovulation, corpus luteum (CL) size and plasma progesterone concentration, 24 female alpacas were synchronised and treated with either: (1) 1 mL 0.9% saline (n = 5); (2) 4 µg buserelin (n = 5); (3) 1 mg β-NGF protein (n = 5); (4) 0.1 mg β-NGF (n = 5); or (5) 0.01 mg β-NGF (n = 4). Females were examined by transrectal ultrasonography at 1–2-h intervals between 20 and 45 h after treatment or until ovulation occurred, as well as on Day 8 to observe the size of the CL, at which time blood was collected to measure plasma progesterone concentrations. Ovulation was detected in 0/5, 5/5, 5/5, 3/5 and 0/4 female alpacas treated with saline, buserelin, 1, 0.1 and 0.01 mg β-NGF, respectively. Mean ovulation interval (P = 0.76), CL diameter (P = 0.96) and plasma progesterone concentration (P = 0.96) did not differ between treatments. Mean ovulation interval overall was 26.2 ± 1.0 h. In conclusion, buserelin and 1 mg β-NGF are equally effective at inducing ovulation in female alpacas, but at doses ≤0.1 mg, β-NGF is not a reliable method for the induction of ovulation.

Item Type: Article
Divisions: Animal Production, Welfare and Veterinary Sciences
Depositing User: Mr Darren Roberts
Date Deposited: 07 Nov 2018 14:52
Last Modified: 29 Nov 2018 12:47
URI: http://hau.repository.guildhe.ac.uk/id/eprint/17113

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