Isolation and identification of anthocyanin component in the fruits of Acanthopanax Sessiliflorus (Rupr. & Maxim.) Seem. by means of high speed counter current chromatography and evaluation of Its antioxidant activity

Chen, L., Xin, X., Feng, H., Li, S., Cao, Q., Wang, X. and Vriesekoop, F. (2020) Isolation and identification of anthocyanin component in the fruits of Acanthopanax Sessiliflorus (Rupr. & Maxim.) Seem. by means of high speed counter current chromatography and evaluation of Its antioxidant activity. Molecules, 25 (8). p. 1781.

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Abstract

Acanthopanax sessiliflorus (Rupr. & Maxim.) Seem. (Araliaceae) is one of the most abundant species of genus Acanthopanax. The fruits of A. sessiliflorus are used in traditional medical protocols as an analgesic, tonic, antidiabetic, antihypertensive, anti-inflammatory, antitumor, and immune-stimulating agent. In this work, we carried out a comprehensive investigation into the anthocyanin components in the fruits of A. sessiliflorus. The anthocyanin content in the fresh fruits of A. sessiliflorus was determined by high performance liquid chromatography-diode array detection (HPLC/DAD), and the anthocyanin component was isolated from these using high-speed counter-current chromatography (HSCCC) and elucidated by electro-spray ionization-mass spectrometry (ESI/MS), 1H- and 13C-NMR. Its antioxidant activity was evaluated by ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH).We found that A. sessiliflorus contained a gross anthocyanin content of 121.35 mg/100 g. HSCCC was successfully used for separation and purification of the primary anthocyanin component, cyanidin 3-xylosyl-galactoside. The antioxidant and radical scavenging tests indicated that cyanidin 3-xylosyl-galactoside is a potent antioxidant.

Item Type: Article
Keywords: anthocyanin, Acanthopanax sessiliflorus (Rupr. & Maxim.) Seem., high-speed counter-current chromatography (HSCCC), antioxidant activity
Divisions: Food Science and Agri-food Supply Chain Management
Depositing User: Ms Kath Osborn
Date Deposited: 01 Jun 2020 15:50
Last Modified: 01 Jun 2020 15:50
URI: http://hau.repository.guildhe.ac.uk/id/eprint/17547

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