An Arduino based automatic pressure evaluation system to quantify growth of non‐model anaerobes in culture

Wilken, S.E., Leggieri, P.A., Kerdman‐Andrade, C., Reilly, M., Theodorou, M.K. and O'Malley, M.A. (2020) An Arduino based automatic pressure evaluation system to quantify growth of non‐model anaerobes in culture. AIChE Journal.

[img] Text
Mike Theodorou an Arduino upload.pdf - Accepted Version
Restricted to Repository staff only until 4 July 2021.

Download (1MB) | Request a copy

Abstract

Measuring the growth rate of non‐model anaerobic microbes typically requires the use of time‐consuming and often destructive manual measurements. Here, an Arduino based automatic pressure evaluation system (A‐APES) was developed to automatically measure the rate of fermentation gas production as a proxy for microbial growth in anaerobic systems. The A‐APES system measures accumulated gas pressure in sealed cultures accurately at high‐resolution, while venting the system at programmed intervals to prevent over pressurization. The utility of A‐APES is demonstrated in this study by quantifying the growth rate and phases of a biomass‐degrading anaerobic gut fungus, which cannot be otherwise measured via conventional techniques due to its association with particulate substrates. Given the utility of the A‐APES approach, we provide a complete construction guide to fabricate the device, which is three times less expensive compared to existing commercial alternatives.

Item Type: Article
Keywords: anaerobic fungi Arduino, automatic culture equipment, non‐model microbe, pressure transducer
Divisions: Crop and Environment Sciences
Depositing User: Ms Kath Osborn
Date Deposited: 28 Aug 2020 12:08
Last Modified: 28 Aug 2020 12:08
URI: https://hau.repository.guildhe.ac.uk/id/eprint/17571

Actions (login required)

Edit Item Edit Item