An improved conjugation method for Pseudomonas syringae

Neale, H.C., Hulin, M.T., Harrison, R.J., Jackson, R.W., Mansfield, J.W. and Arnold, D.L. (2020) An improved conjugation method for Pseudomonas syringae. Journal of Microbiological Methods, 177 (106025).

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In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required increase in frequency and facilitated conjugation of otherwise recalcitrant strains.

Item Type: Article
Keywords: Pseudomonas, Conjugation, TnSeq
Depositing User: Ms Kath Osborn
Date Deposited: 28 Aug 2020 13:22
Last Modified: 11 Aug 2021 03:30

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