Characterization of canine intestinal microRNA expression in inflammatory bowel disease and T-cell lymphoma.
Irving, J. R., Hiron, T. K., Davison, L. J., Xia, D., Beck, S., Werling, D. and Williams, J. (2023) Characterization of canine intestinal microRNA expression in inflammatory bowel disease and T-cell lymphoma. Journal of Comparative Pathology, 204. pp. 23-29. ISSN 00219975
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Irving, J.R. Characterization of canine intestinal microRNA UPLOAD OCR..docx.pdf - Published Version Available under License Creative Commons Attribution. Download (2MB) | Preview |
Abstract
Differentiating between canine inflammatory bowel disease (IBD) and intestinal T-cell lymphoma by histopathological examination of endoscopically-derived intestinal biopsies can be challenging and involves an invasive procedure requiring specialized equipment and training. A rapid, non-invasive method of diagnosis, such as blood or faecal analysis for a conserved and stable biomarker, would be a useful adjunct or replacement. Studies on dogs and humans with various types of lymphoma have shown altered microRNA (miRNA) expression patterns in blood, faeces and tissues indicating their potential use as biomarkers of disease. The present study used residual archived endoscopically-derived, formalin-fixed, paraffin-embedded (FFPE) duodenal tissue taken from pet dogs undergoing routine investigation of gastrointestinal disease. The dogs had previously been diagnosed with either normal/minimal intestinal inflammation, severe IBD or intestinal T-cell lymphoma. Next generation sequencing with qPCR validation was used to elucidate differentially expressed miRNAs between groups. Our results show that miRNA can be extracted from archived endoscopically-derived FFPE tissues from the canine duodenum and used to differentiate normal/minimally inflamed canine duodenal tissue from severe lymphoplasmacytic IBD and T-cell lymphoma.
| Item Type: | Article |
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| Keywords: | dog, gastrointestinal, lymphoma, microRNA, T cell. |
| Divisions: | Animal Health, Behaviour and Welfare (from 1.09.21) |
| Depositing User: | Miss Anna Cope |
| Date Deposited: | 28 Jul 2023 12:56 |
| Last Modified: | 28 Jul 2023 12:56 |
| URI: | https://hau.repository.guildhe.ac.uk/id/eprint/17982 |
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