Scents and sensibility: Best practice in insect olfactometer bioassays

Roberts, J.M., Clunie, B.J., Leather, S.R., Harris, W.E. and Pope, T.W. (2023) Scents and sensibility: Best practice in insect olfactometer bioassays. Entomologia Experimentalis et Applicata. ISSN 0013-8703

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Olfactometers have been used for more than 100 years and are integral to experimental chemical ecology. Studies utilising olfactometer bioassays form the foundation for understanding the behavioural responses of invertebrates to chemical stimuli under standardised laboratory conditions. Widely used olfactometry apparatuses include two-arm olfactometers for binary responses through to four- and six-arm arenas to evaluate more complex behaviours. Despite its prevalence in chemical ecology studies, there has never been a review of experimental best practice in olfactometry. This review critically evaluates both olfactometry methods and applications as well as experimental design and analysis. We aim to outline a standard of good practice to improve experimental design and reporting for studies involving olfactometry, thereby establishing a reference guide to build a robust experimental workflow for olfactometry bioassays.

Item Type: Article
Keywords: behaviour, binary response, bioassay, chemical ecology, chemical stimuli, complex behaviours, experimental design, olfactometer, reference guide, standard of good practice, statistical analysis, volatile compounds
Divisions: Agriculture and Environment (from 1.08.20)
Depositing User: Miss Terésa-Marie Jones
Date Deposited: 03 Aug 2023 10:41
Last Modified: 03 Aug 2023 10:41

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