Optimization of phytase production from escherichia coli by altering solids-state fermentation conditions
McKinney, K., Combs, J., Becker, P., Humphries, A., Filer, K. and Vriesekoop, F. (2015) Optimization of phytase production from escherichia coli by altering solids-state fermentation conditions. Fermentation, 1 (1). pp. 13-23.
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Andrea Humphries Optimization of phytase production.pdf - Published Version Available under License Creative Commons Attribution. Download (403kB) | Preview |
Abstract
Cultivation of Escherichia coli on wheat-bran substrate under various Solid-State Fermentation (SSF) conditions was evaluated for phytase yield along with the enzyme activity profile as a potential, low-cost alternative to submerged-liquid fermentation. The maximum phytase activity achieved by E. coli was 350 ± 50 SPU of phytase activity per gram of bran, incubated for 96 h with a substrate bed moisture content of 70% (w/v) at 37 °C with a relative air humidity of 90%, and supplemented with 10% (w/w bran) Luria-Bertani broth powder which translates into a 300% increase in phytase activity compared with an un-supplemented culture. The greatest improvements in phytase yield were associated with nutrient supplementation and the optimization of initial substrate moisture content. E. coli production of phytase utilizing solid-state fermentation technology was shown to be feasible utilizing the low-cost agro-residue wheat bran as substrate. Furthermore, the effect of pH and temperature on phytase activity was monitored from pH 2.5 to pH 7.5, and for temperatures ranging from 20 °C to 70 °C. Optimal phytase activity was at pH 5.5 and 50 °C when produced under the SSF optimized conditions.
| Item Type: | Article |
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| Keywords: | E. coli, enzyme, optimization, phytase, solid state fermentation |
| Divisions: | Food, Land and Agribusiness Management |
| Depositing User: | Ms Kath Osborn |
| Date Deposited: | 09 Feb 2017 20:07 |
| Last Modified: | 22 Jul 2021 11:31 |
| URI: | https://hau.repository.guildhe.ac.uk/id/eprint/15198 |
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