Methods for genomic characterization and maintenance of anaerobic fungi
Peng, X., Swift, C.L., Theodorou, M.K. and O'Malley, M.A. (2018) Methods for genomic characterization and maintenance of anaerobic fungi. In: Fungal genomics: methods and protocols. Springer, New York, pp. 53-67.
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Abstract
The rapid development of molecular biology and bioinformatics has fueled renewed interests in anaerobic fungi from the phylum Neocallimastigomycota. This chapter presents well-established methods for isolation, routine cultivation, and cryopreservation of anaerobic fungi. Moreover, detailed nucleic acid extraction protocols are provided, which should enable readers to isolate high-quality DNA and RNA from a variety of anaerobic fungal culture media for downstream applications such as next-generation sequencing.
| Item Type: | Book Section |
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| Keywords: | Anaerobic fungi, Genomes, Transcriptomes, DNA extraction, RNA extraction, Cultivation, Isolatio, n Neocallimastigomycota, Next-generation sequencing, Consecutive batch culture |
| Divisions: | Animal Production, Welfare and Veterinary Sciences (to 31.07.20) |
| Depositing User: | Ms Kath Osborn |
| Date Deposited: | 08 Jul 2019 13:27 |
| Last Modified: | 07 Jun 2020 04:10 |
| URI: | https://hau.repository.guildhe.ac.uk/id/eprint/17424 |
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