Identification of genetic loci in lettuce mediating quantitative resistance to fungal pathogens
Pink, H., Talbot, A., Graceson, A., Graham, J., Higgins, G., Taylor, A., Jackson, A.C., Truco, M., Michelmore, R., Yao, C., Gawthrop, F., Pink, D.A.C., Hand, P., Clarkson, J.P. and Denby, K. (2022) Identification of genetic loci in lettuce mediating quantitative resistance to fungal pathogens. Theoretical and Applied Genetics, 135 (7). pp. 2481-2500.
|
Text
David Pink Identification of genetic UPLOAD.OCR.pdf - Published Version Available under License Creative Commons Attribution. Download (6MB) | Preview |
Abstract
Lactuca sativa L. (lettuce) is an important leafy vegetable crop grown and consumed globally. Chemicals are routinely used to control major pathogens, including the causal agents of grey mould (Botrytis cinerea) and lettuce drop (Sclerotinia sclerotiorum). With increasing prevalence of pathogen resistance to fungicides and environmental concerns, there is an urgent need to identify sources of genetic resistance to B. cinerea and S. sclerotiorum in lettuce. We demonstrated genetic variation for quantitative resistance to B. cinerea and S. sclerotiorum in a set of 97 diverse lettuce and wild relative accessions, and between the parents of lettuce mapping populations. Transcriptome profiling across multiple lettuce accessions enabled us to identify genes with expression correlated with resistance, predicting the importance of post-transcriptional gene regulation in the lettuce defence response. We identified five genetic loci influencing quantitative resistance in a F6 mapping population derived from a Lactuca serriola (wild relative) × lettuce cross, which each explained 5–10% of the variation. Differential gene expression analysis between the parent lines, and integration of data on correlation of gene expression and resistance in the diversity set, highlighted potential causal genes underlying the quantitative trait loci.
| Item Type: | Article |
|---|---|
| Divisions: | Agriculture and Environment (from 1.08.20) |
| Depositing User: | Mrs Rachael Giles |
| Date Deposited: | 13 Oct 2022 15:23 |
| Last Modified: | 13 Oct 2022 15:23 |
| URI: | https://hau.repository.guildhe.ac.uk/id/eprint/17888 |
Actions (login required)
 |
Edit Item |